Electrophoresis
Sample Identification
Giant Instant Non-Fat Dry Milk and Casein Protein
Purpose
The purpose of this experiment was to separate and determine the molecular weight of two protein samples by a BioRad protein assay method running a polyacrylamide gel electrophoresis (PAGE) using sodium dodecyl sulfate (SDS).
Results
The casein protein appeared to have less bands and the darker bands were between 15 to 75 kD. The milk sample showed many bands ranging from 10 kD to just about 250 kD. Both samples showed very dark bands at around 25 to 37 kD.
Comments
Our polyacrylamide gels did not produce a good resolution, therefore, making it difficult to determine where the bands were located. This could have been cause by the length of time the gel was held in the staining solution. Another possible cause of the low resolution is not allowing the gel to destain for a proper amount of time. However, our results for Casein protein seemed to be in the range of the known 10 to 100 kD Casein molecular weight. Also our results for milk seemed to be accurate as it had a wide range of molecular bands indicating all the milk system components.
Our polyacrylamide gels did not produce a good resolution, therefore, making it difficult to determine where the bands were located. This could have been cause by the length of time the gel was held in the staining solution. Another possible cause of the low resolution is not allowing the gel to destain for a proper amount of time. However, our results for Casein protein seemed to be in the range of the known 10 to 100 kD Casein molecular weight. Also our results for milk seemed to be accurate as it had a wide range of molecular bands indicating all the milk system components.